show Abstracthide AbstractHospital acquired infections (HAIs) pose a serious threat to patients. Accordingly, hospitals spend a large amount of time and money trying to prevent them. Many HAIs are due to contamination from workers hands and contact with high-touch surfaces. Therefore, we set out to test the efficacy of a new preventative technology, AIONX Antimicrobial Technologies, Incs cleanSURFACES, which is designed to continuously prevent re-colonization of cleaned surfaces. To that end, we swabbed surfaces before (Pre-Baseline) and after (Post) application of the cleanSURFACES at various timepoints (Day 1, Day 7, and Day 28). Previous studies have examined the hospital microbiome using the 16S rRNA gene. However, here, the swabs were processed for metatranscriptomic analysis to allow for greater taxonomic resolution and the detection of fungi and viruses. Overall, there was a global reduction in microbial diversity in Post samples. Additionally, Post samples clustered together much more closely than baseline samples, suggesting a defined shift after application in most cases. This shift was characterized by a general depletion of most microbes, as the entire domain Bacteria was significantly reduced in Post samples, with multiple phyla also being reduced over the duration of the study. Notably, specific pathogens of interest, including Staphylococcus aureus and Streptococcus, were depleted too. Taken together, these findings strongly indicate that cleanSURFACES effectively inhibit colonization by a wide variety of microbes, including many important pathogens.